A second hand, electrically powered autoclave ( Steriliser) with a sterilising chamber 350mm high x 350 mm wide x 750 mm deep with 2 x 3500 watt, 240 volt heater coils set in an open evaporator. It requires 2 separate 15 amp, 240 volt supplies to operate. Suited to autoclaving fluids or for sterilising dressings – no drying cycle. Features selectable sterilising temperature and selectable sterilisation times. The chamber of this autoclave was built in 1971. It was incorporated in the frame and commissioned 11/2/1986. It was used up to once a fortnight to sterilise flasks of orchid tissue culture medium commencing 1986 for about 3 years and not used again until January 2021when I upgraded plumbing and control systems. It is best run with Distilled or Rain Water – Rain Water is much cheaper!
It has a header tank to conveniently provide water for the sterilising cycles and there is a ‘Water’ valve to allow filling of the Evaporator Pan from the Header Tank. There is also a ‘Waste’ valve to allow draining of the Header Tank or the Evaporator Pan if desired. A hose connection is provided for convenient waste discharge.
It has an Overheat / Overpressure Thermostat to prevent the machine reaching dangerous temperatures and pressures. It has an adjustable Thermostat to select, set and control the operating temperature of the Chamber – Warning DO NOT EXCEED 135 deg C !!!!
It has an adjustable Timer with adjustable settings of hours, minutes etc that will cut in when the predetermined operating temperature has been reached and cut out after the preset time at that temperature has been achieved.
It has additional circuitry to assure the Heaters remain off after the predetermined sterilising time has passed to avoid over-cook and to thus maximise media potency.
It has additional circuitry and components to automatically allow the contents of the Evaporator Pan and the steam of the Chamber to be controllably vented via the Header Tank. The rate of discharge from the Autoclave Chamber to the header tank is via a Steam Solenoid and an In-Line Cock – which should be adjusted so that the chamber will vent to about zero chamber pressure over about 10-12 minutes which allows the medium in the autoclaved flasks to cool slowly without vigorous, splatter-boiling during depressurisation .
It also has additional circuitry added by way of a manually set, mechanical timer which enables the operator to silence the buzzer which has sounded to indicate that sterilisation has occurred and that the autoclave is now automatically venting / depressurising . When it sounds, the operator should immediately set the manual timer dial to 10 – 12 minutes which time should be sufficient to let the autoclave automatically depressurise to at or near zero chamber pressure which is safe to open the autoclave door and remove its load. DON’T ATEMPT TO OPEN DOOR UNLESS CHAMBER PRESSURE IS AT OR CLOSE TO ZERO!!!!! The autoclave is very efficient and even more efficient if used to do several to many loads in sequence whilst it is still hot. This efficiency is further enhanced if the header tank holds not more than about one and a half evaporator pans of water. At the end of each sterilising cycle, the remaining heated water in the evaporator pan is expelled to the header tank and when readmitted after to the evaporator pan for the next load, starts off hot and lessens the energy needed to complete each subsequent cycle. The unit is very quick to heat up and so is very convenient to use.
It will hold 27 x 750mL flasks, about 70 x 500mL flasks and many more 375mL or 250mL flasks.
I estimate it weighs between 200 & 250 kg. Able to be transported / moved by two persons using the likes of a Mitsubishi L300 Van or trailer. I have offloaded it from a 7 x 4 trailer and moved it into my shed on my own. Transport may be possible using Removalists – such transport ( if required ) to be arranged by negotiation at cost to purchaser.