Media Preparation

Media Preparation 2017-05-05T10:33:58+00:00

There are many ways in which media can be prepared. If you have a good method already you should continue to use it.

  1. Heat a litre of distilled water to boiling in a very clean container (we use an electric kitchen kettle)
  2. Immediately add about 250mI, of the boiled water to a blender and immediately empty the contents of a 1L sachet of medium
  3. Replace the blender lid firmly, and pulse the blender quickly 2-3 times to start mixing the contents & heat the air above the water without blowing the lid off. Be very carefully to avoid scaling yourself
  4. Now run the blender for 10 to 20 seconds for a thorough mix.
  5. Pour the mixture into your measuring vessel and rinse all the thick mixture from the blender into the measuring vessel with some more boiled water
  6. If banana pulp is required, weigh the banana and microwave it for 60 seconds on high. Add it to the blender with some hot distilled water and blend to a fine pulp then add it to the measuring vessel
  7. If using coconut water add that now
  8. Add the remaining hot water to make up about half the required volume. Make up the remaining volume with cold distilled water
  9. Mix thoroughly before pouring the mix into your flasks

Note: When pouring, avoid spilling any medium on to the upper neck or outer threaded area. This will prevent bacteria or fungi from having a path to grow into your flasks.

Make sure the flasks/vessels and their lids can withstand the heat of pressure cooking or autoclaving to sterilise them and their contents.